Understanding the Neurobiological Basis of Behavior: A Good Way to Go

in humans through a comprehensive number of neurobiological approaches (including lesions, brain stimulation, neuroimaging, human brain banks, and genetic analyses – from single-nucleotide polymorphisms to genome – and epigenome – wide analyses) complementing progressively refined behavioral analyses [with virtual reality approaches already being an actual possibility to increase the ecological value of behavioral testing (Slater et al., 2006)]. In fact, the question ‘why do we need mice if nowadays we can virtually do everything in humans?’ is becoming not so infrequent. Even though answering to the question is not an easy task, studies on animals have still much to offer to the understanding of the neurobiological basis of behavior. In no way, human researchers can dream to manipulate variables and conditions and to get to the fine detailed cellular, molecular, and functional approaches in the studies that animal research allows. In fact, the good shape of the two subfields (animal and human Behavioral Neuroscience) is clearly materializing in mutual benefit (Carola et al., 2008; de Quervain et al., 2007) and the reciprocal interaction comes as a momentum for the field as a whole. Let’s now focus on what still seems to be the core study in the field of Behavioral Neuroscience: animal and, to a large extent, rodent studies. What are the challenges we are facing now? Being aware that any attempt to answer such a question can only be subjective, I will briefly comment on the issues that I am particularly sensitive to, without any intention to be comprehensive. The first issue is certainly the urgent need for a refining of behavioral testing, and includes many issues from continuing improvement of the accuracy of measures to progressively automatizing the whole testing process. One idea would be to develop more complex housing environments including a range of testing opportunities under social conditions, with the main goals being to avoid taking out animals from their normal environment, minimizing differences due to human manipulation, and improvement in standardization. Although there is already an interesting development in the market, known as the Intellicage (Knapska et al., 2006), there is still huge room for expansion. Refinement of the behavioral testing would also include the development of new validated tests to enhance and improve the measurement of behavior. There is also an imminent need for improving and expanding the animal models of psychopathology. The development of models with good face validity can greatly benefit from automated approaches (taking out the animal from its home cage for a restricted time per day to be submitted, for example, to stress-induction procedures with the aim of eventually modeling depression is clearly less ‘ecologically’ valid than organizing the delivery of stressors in their ‘natural’ environment according to a controlled scheme). Considering the sophistication of analyses reached at other domains of Neuroscience (such as the high resolution of current Not so long ago, Behavioral Neuroscience was considered a ‘soft’ science, with the search for new molecules and the cellular functions of the molecules being the leading model illustrating what was considered essential to be studied to understand brain function. This has drastically changed in the last decade, particularly with the publication of the human genome and later on that of other species (notably the mouse one). The time came when the need for placing molecules in ‘context’ and identifying the role of the molecules in the behaving animal became clearly apparent. Such a development in the Neuroscience panorama has placed Behavioral Neuroscience at quite a central place. It is clear now that we cannot determine whether a particular molecule is involved in memory formation just by testing the molecule’s effects through in vitro approaches (by evaluating, for example, whether it influences neurite outgrowth in neuronal cultures, or long-term potentiation (LTP) in brain slices, just to mention two of the approaches most frequently used in this type of screening). Although the earlier studies showed that effects on learning should match the effects found for LTP, nowadays there are many examples in the literature of dissociations between the effects exerted on LTP and on memory tasks by specific molecules, most frequently with one (LTP or learning) being affected and not the other (Shimshek et al., 2006), or even each of them displaying opposite effects (Rutten et al., 2008). In fact, the lack of prediction from one system to the other makes total sense. Reproduction of the dynamic complexity that characterizes every single organism in a piece of brain tissue now appears to be a naïve oversimplification. Many are the missing elements (hormones, cytokines, to only cite a few, well established ones), that play a role in behavior and cognition in the entire animal when only a piece of tissue is examined. However, proposing a central role for Behavioral Neuroscience should not disregard the fact that Human Neuroscience is taking strong positions in the domains that have been classically defined within the scope of Behavioral Neuroscience. The non-inclusion of human studies in the field of Behavioral Neuroscience is, in my view, an artificial segregation. As stated in the mission statement of Frontiers in Behavioral Neuroscience, I consider that this field includes the study of the neurobiological basis of behavior both in animals and humans. Now, we can be informed of the neurobiological basis of behavior